ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mRNA expressions of RASSF1A, Galectin-3 and TPO in papillary thyroid carcinoma and some other thyroid benign lesions, and evaluate their diagnostic significance.</p><p><b>METHODS</b>Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of RASSF1A, galectin-3 and TPO in the samples from 73 cases, including 23 cases with papillary thyroid cancer, 16 with nodular goiter, 29 with thyroid adenoma and 5 with Hashimoto's disease.</p><p><b>RESULTS</b>A statistically significant difference in the mRNA expression of RASSF1A, Galectin-3 and TPO was observed between papillary thyroid carcinoma and follicular benign lesions (P<0.05). However, there was no significant difference among various kinds of benign lesions (P>0.05). A negative correlation of the expression of RASSF1A and Galectin-3 mRNA was found between thyroid benign lesions and malignant ones (P = 0.000). While the mRNA expression of RASSF1A and TPO was positively correlated between benign and malignant lesions (P = 0.028).</p><p><b>CONCLUSION</b>Loss of expression of RASSF1A and TPO mRNA but high expression of Galectin-3 mRNA in papillary thyroid carcinoma are common. Therefore, the products of these three genes may be closely related to the development of thyroid papillary carcinoma, and may be used as useful markers in differential diagnosis of papillary thyroid carcinoma from the benign lesions. The results are more reliable if this detection method is used in combination with other techniques.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Autoantigens , Genetics , Metabolism , Biomarkers, Tumor , Metabolism , Carcinoma, Papillary , Genetics , Metabolism , Pathology , Diagnosis, Differential , Galectin 3 , Genetics , Metabolism , Goiter, Nodular , Genetics , Metabolism , Pathology , Hashimoto Disease , Genetics , Metabolism , Pathology , Iodide Peroxidase , Genetics , Metabolism , Iron-Binding Proteins , Genetics , Metabolism , RNA, Messenger , Metabolism , Thyroid Neoplasms , Genetics , Metabolism , Pathology , Tumor Suppressor Proteins , Genetics , MetabolismABSTRACT
Objective: To detect the expression of human stanniocalcin-1 (hSTC-1) mRNA in peripheral blood and tumor tissues of breast cancer patients, determine micrometastases in peripheral blood, and study the relationship between the micrometastases and malignant degree of breast cancer. Methods: The expression of hSTC-1 mRNA in peripheral blood and breast cancer tissues was detected by RT-PCR. The expression of estrogen receptor (ER), progesterone receptor (PR), and vascular endothelial growth factor (VEGF) in breast cancer tissues were detected by immunohistochemical method. Results: Expression of hSTC-1 mRNA was identified in breast cancer tissues from 28 of 30 patients (93.3%) and in peripheral blood from 19 of 51 patients (37.3%). Expression of hSTC-1 mRNA was not detected in the peripheral blood from 17 women without breast cancer. Expression of hSTC-1 mRNA in the peripheral blood significantly correlated with multiple clinicopathological factors (P < 0.05), including tumor size, metastasis of lymph nodes, TNM stage, and the expression of ER and VEGF. Conclusion: The expression of hSTC-1 mRNA in the peripheral blood of breast cancer patients suggests the occurrence of micrometastasis. It significantly correlates with multiple clinicopathological factors which determined the prognosis of breast cancer. So hSTC-1 is proposed as a new marker for detecting micrometastasis of breast cancer in the peripheral blood.
ABSTRACT
Objective To study the protective effect of total flavones of hawthorn leaf (TFHL) on renal ischemia/reperfusion (I/R) in rats.Methods A model of renal I/R was made by clamping double renal pedical for 60 min and reperfusion for 24 h.TFHL (30 or 60 mg/kg) was injected before clamping renal ischemia. The level of serum urea nitrogen(BUN), serum creatinine(Scr), tumor necrosis factor-?(TNF-?),interleukin-1(IL-1) in serum were detected after reperfusion for 1 and 24 h,respectively,and the level of malonaldehyde (MDA) and erythrocuprein (SOD) in renal tissues were measured after reperfusion for 24 h.Results TFHL significantly decreased the level of BUN and Scr, decreased the concentration of TNF-? and IL-1 in serum. Pretreatment with TFHL also enhanced the activity of SOD reduced the concentration of MDA in renal after I/R.Conclusions TFHL has significantly protective effect on renal I/R and markedly improve the renal function. The mec-hanisms are associated with decreasing the lipid peroxidation reaction and reducing the level of TNF-? and IL-1.